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normal human mammary epithelial cells humec  (ATCC)


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    ATCC normal human mammary epithelial cells humec
    Normal Human Mammary Epithelial Cells Humec, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1327 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mammary+epithelial+cells+humecs/Primary+Mammary+Epithelial+Cells%3B+Normal%2C+Human/pm37387248-166-10-20
    Average 99 stars, based on 1327 article reviews
    normal human mammary epithelial cells humec - by Bioz Stars, 2026-10
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    Related Articles

    Amplification:

    Article Title: The oncogene AAMDC links PI3K-AKT-mTOR signaling with metabolic reprograming in estrogen receptor-positive breast cancer
    Article Snippet: The MCF-12A cells were cultured in 50% Ham’s F12 medium and 50% DMEM medium containing 5% horse serum, 1% antibiotic-antimycotic and 20 ng/mL epidermal growth factor (EGF), 10 μg/mL insulin, 500 ng/mL hydrocortisone and 100 ng/mL of cholera toxin.The MCF-12A cells were cultured in 50% Ham’s F12 medium and 50% DMEM medium containing 5% horse serum, 1% antibiotic-antimycotic and 20 ng/mL epidermal growth factor (EGF), 10 μg/mL insulin, 500 ng/mL hydrocortisone and 100 ng/mL of cholera toxin.. For experiments involving non-genetically manipulated human mammary epithelial cells (HuMECs) (Fig. ), human primary mammary epithelial cells were purchased from ATCC (HMEC, PCS-600-010).. These cells were grown in Mammary Epithelial Cell Basal Medium (ATCC) supplemented with the components of the Mammary Epithelial Cell Growth Kit (ATCC).These cells were grown in Mammary Epithelial Cell Basal Medium (ATCC) supplemented with the components of the Mammary Epithelial Cell Growth Kit (ATCC).

    Expressing:

    Article Title: The oncogene AAMDC links PI3K-AKT-mTOR signaling with metabolic reprograming in estrogen receptor-positive breast cancer
    Article Snippet: The MCF-12A cells were cultured in 50% Ham’s F12 medium and 50% DMEM medium containing 5% horse serum, 1% antibiotic-antimycotic and 20 ng/mL epidermal growth factor (EGF), 10 μg/mL insulin, 500 ng/mL hydrocortisone and 100 ng/mL of cholera toxin.The MCF-12A cells were cultured in 50% Ham’s F12 medium and 50% DMEM medium containing 5% horse serum, 1% antibiotic-antimycotic and 20 ng/mL epidermal growth factor (EGF), 10 μg/mL insulin, 500 ng/mL hydrocortisone and 100 ng/mL of cholera toxin.. For experiments involving non-genetically manipulated human mammary epithelial cells (HuMECs) (Fig. ), human primary mammary epithelial cells were purchased from ATCC (HMEC, PCS-600-010).. These cells were grown in Mammary Epithelial Cell Basal Medium (ATCC) supplemented with the components of the Mammary Epithelial Cell Growth Kit (ATCC).These cells were grown in Mammary Epithelial Cell Basal Medium (ATCC) supplemented with the components of the Mammary Epithelial Cell Growth Kit (ATCC).

    Gene Expression:

    Article Title: The oncogene AAMDC links PI3K-AKT-mTOR signaling with metabolic reprograming in estrogen receptor-positive breast cancer
    Article Snippet: The MCF-12A cells were cultured in 50% Ham’s F12 medium and 50% DMEM medium containing 5% horse serum, 1% antibiotic-antimycotic and 20 ng/mL epidermal growth factor (EGF), 10 μg/mL insulin, 500 ng/mL hydrocortisone and 100 ng/mL of cholera toxin.The MCF-12A cells were cultured in 50% Ham’s F12 medium and 50% DMEM medium containing 5% horse serum, 1% antibiotic-antimycotic and 20 ng/mL epidermal growth factor (EGF), 10 μg/mL insulin, 500 ng/mL hydrocortisone and 100 ng/mL of cholera toxin.. For experiments involving non-genetically manipulated human mammary epithelial cells (HuMECs) (Fig. ), human primary mammary epithelial cells were purchased from ATCC (HMEC, PCS-600-010).. These cells were grown in Mammary Epithelial Cell Basal Medium (ATCC) supplemented with the components of the Mammary Epithelial Cell Growth Kit (ATCC).These cells were grown in Mammary Epithelial Cell Basal Medium (ATCC) supplemented with the components of the Mammary Epithelial Cell Growth Kit (ATCC).

    Microarray:

    Article Title: The oncogene AAMDC links PI3K-AKT-mTOR signaling with metabolic reprograming in estrogen receptor-positive breast cancer
    Article Snippet: The MCF-12A cells were cultured in 50% Ham’s F12 medium and 50% DMEM medium containing 5% horse serum, 1% antibiotic-antimycotic and 20 ng/mL epidermal growth factor (EGF), 10 μg/mL insulin, 500 ng/mL hydrocortisone and 100 ng/mL of cholera toxin.The MCF-12A cells were cultured in 50% Ham’s F12 medium and 50% DMEM medium containing 5% horse serum, 1% antibiotic-antimycotic and 20 ng/mL epidermal growth factor (EGF), 10 μg/mL insulin, 500 ng/mL hydrocortisone and 100 ng/mL of cholera toxin.. For experiments involving non-genetically manipulated human mammary epithelial cells (HuMECs) (Fig. ), human primary mammary epithelial cells were purchased from ATCC (HMEC, PCS-600-010).. These cells were grown in Mammary Epithelial Cell Basal Medium (ATCC) supplemented with the components of the Mammary Epithelial Cell Growth Kit (ATCC).These cells were grown in Mammary Epithelial Cell Basal Medium (ATCC) supplemented with the components of the Mammary Epithelial Cell Growth Kit (ATCC).

    Immunohistochemistry:

    Article Title: The oncogene AAMDC links PI3K-AKT-mTOR signaling with metabolic reprograming in estrogen receptor-positive breast cancer
    Article Snippet: The MCF-12A cells were cultured in 50% Ham’s F12 medium and 50% DMEM medium containing 5% horse serum, 1% antibiotic-antimycotic and 20 ng/mL epidermal growth factor (EGF), 10 μg/mL insulin, 500 ng/mL hydrocortisone and 100 ng/mL of cholera toxin.The MCF-12A cells were cultured in 50% Ham’s F12 medium and 50% DMEM medium containing 5% horse serum, 1% antibiotic-antimycotic and 20 ng/mL epidermal growth factor (EGF), 10 μg/mL insulin, 500 ng/mL hydrocortisone and 100 ng/mL of cholera toxin.. For experiments involving non-genetically manipulated human mammary epithelial cells (HuMECs) (Fig. ), human primary mammary epithelial cells were purchased from ATCC (HMEC, PCS-600-010).. These cells were grown in Mammary Epithelial Cell Basal Medium (ATCC) supplemented with the components of the Mammary Epithelial Cell Growth Kit (ATCC).These cells were grown in Mammary Epithelial Cell Basal Medium (ATCC) supplemented with the components of the Mammary Epithelial Cell Growth Kit (ATCC).

    In Situ:

    Article Title: The oncogene AAMDC links PI3K-AKT-mTOR signaling with metabolic reprograming in estrogen receptor-positive breast cancer
    Article Snippet: The MCF-12A cells were cultured in 50% Ham’s F12 medium and 50% DMEM medium containing 5% horse serum, 1% antibiotic-antimycotic and 20 ng/mL epidermal growth factor (EGF), 10 μg/mL insulin, 500 ng/mL hydrocortisone and 100 ng/mL of cholera toxin.The MCF-12A cells were cultured in 50% Ham’s F12 medium and 50% DMEM medium containing 5% horse serum, 1% antibiotic-antimycotic and 20 ng/mL epidermal growth factor (EGF), 10 μg/mL insulin, 500 ng/mL hydrocortisone and 100 ng/mL of cholera toxin.. For experiments involving non-genetically manipulated human mammary epithelial cells (HuMECs) (Fig. ), human primary mammary epithelial cells were purchased from ATCC (HMEC, PCS-600-010).. These cells were grown in Mammary Epithelial Cell Basal Medium (ATCC) supplemented with the components of the Mammary Epithelial Cell Growth Kit (ATCC).These cells were grown in Mammary Epithelial Cell Basal Medium (ATCC) supplemented with the components of the Mammary Epithelial Cell Growth Kit (ATCC).

    Staining:

    Article Title: The oncogene AAMDC links PI3K-AKT-mTOR signaling with metabolic reprograming in estrogen receptor-positive breast cancer
    Article Snippet: The MCF-12A cells were cultured in 50% Ham’s F12 medium and 50% DMEM medium containing 5% horse serum, 1% antibiotic-antimycotic and 20 ng/mL epidermal growth factor (EGF), 10 μg/mL insulin, 500 ng/mL hydrocortisone and 100 ng/mL of cholera toxin.The MCF-12A cells were cultured in 50% Ham’s F12 medium and 50% DMEM medium containing 5% horse serum, 1% antibiotic-antimycotic and 20 ng/mL epidermal growth factor (EGF), 10 μg/mL insulin, 500 ng/mL hydrocortisone and 100 ng/mL of cholera toxin.. For experiments involving non-genetically manipulated human mammary epithelial cells (HuMECs) (Fig. ), human primary mammary epithelial cells were purchased from ATCC (HMEC, PCS-600-010).. These cells were grown in Mammary Epithelial Cell Basal Medium (ATCC) supplemented with the components of the Mammary Epithelial Cell Growth Kit (ATCC).These cells were grown in Mammary Epithelial Cell Basal Medium (ATCC) supplemented with the components of the Mammary Epithelial Cell Growth Kit (ATCC).

    One-tailed Test:

    Article Title: The oncogene AAMDC links PI3K-AKT-mTOR signaling with metabolic reprograming in estrogen receptor-positive breast cancer
    Article Snippet: The MCF-12A cells were cultured in 50% Ham’s F12 medium and 50% DMEM medium containing 5% horse serum, 1% antibiotic-antimycotic and 20 ng/mL epidermal growth factor (EGF), 10 μg/mL insulin, 500 ng/mL hydrocortisone and 100 ng/mL of cholera toxin.The MCF-12A cells were cultured in 50% Ham’s F12 medium and 50% DMEM medium containing 5% horse serum, 1% antibiotic-antimycotic and 20 ng/mL epidermal growth factor (EGF), 10 μg/mL insulin, 500 ng/mL hydrocortisone and 100 ng/mL of cholera toxin.. For experiments involving non-genetically manipulated human mammary epithelial cells (HuMECs) (Fig. ), human primary mammary epithelial cells were purchased from ATCC (HMEC, PCS-600-010).. These cells were grown in Mammary Epithelial Cell Basal Medium (ATCC) supplemented with the components of the Mammary Epithelial Cell Growth Kit (ATCC).These cells were grown in Mammary Epithelial Cell Basal Medium (ATCC) supplemented with the components of the Mammary Epithelial Cell Growth Kit (ATCC).

    Fluorescence:

    Article Title: The oncogene AAMDC links PI3K-AKT-mTOR signaling with metabolic reprograming in estrogen receptor-positive breast cancer
    Article Snippet: The MCF-12A cells were cultured in 50% Ham’s F12 medium and 50% DMEM medium containing 5% horse serum, 1% antibiotic-antimycotic and 20 ng/mL epidermal growth factor (EGF), 10 μg/mL insulin, 500 ng/mL hydrocortisone and 100 ng/mL of cholera toxin.The MCF-12A cells were cultured in 50% Ham’s F12 medium and 50% DMEM medium containing 5% horse serum, 1% antibiotic-antimycotic and 20 ng/mL epidermal growth factor (EGF), 10 μg/mL insulin, 500 ng/mL hydrocortisone and 100 ng/mL of cholera toxin.. For experiments involving non-genetically manipulated human mammary epithelial cells (HuMECs) (Fig. ), human primary mammary epithelial cells were purchased from ATCC (HMEC, PCS-600-010).. These cells were grown in Mammary Epithelial Cell Basal Medium (ATCC) supplemented with the components of the Mammary Epithelial Cell Growth Kit (ATCC).These cells were grown in Mammary Epithelial Cell Basal Medium (ATCC) supplemented with the components of the Mammary Epithelial Cell Growth Kit (ATCC).

    In Situ Hybridization:

    Article Title: The oncogene AAMDC links PI3K-AKT-mTOR signaling with metabolic reprograming in estrogen receptor-positive breast cancer
    Article Snippet: The MCF-12A cells were cultured in 50% Ham’s F12 medium and 50% DMEM medium containing 5% horse serum, 1% antibiotic-antimycotic and 20 ng/mL epidermal growth factor (EGF), 10 μg/mL insulin, 500 ng/mL hydrocortisone and 100 ng/mL of cholera toxin.The MCF-12A cells were cultured in 50% Ham’s F12 medium and 50% DMEM medium containing 5% horse serum, 1% antibiotic-antimycotic and 20 ng/mL epidermal growth factor (EGF), 10 μg/mL insulin, 500 ng/mL hydrocortisone and 100 ng/mL of cholera toxin.. For experiments involving non-genetically manipulated human mammary epithelial cells (HuMECs) (Fig. ), human primary mammary epithelial cells were purchased from ATCC (HMEC, PCS-600-010).. These cells were grown in Mammary Epithelial Cell Basal Medium (ATCC) supplemented with the components of the Mammary Epithelial Cell Growth Kit (ATCC).These cells were grown in Mammary Epithelial Cell Basal Medium (ATCC) supplemented with the components of the Mammary Epithelial Cell Growth Kit (ATCC).

    Quantitative RT-PCR:

    Article Title: The oncogene AAMDC links PI3K-AKT-mTOR signaling with metabolic reprograming in estrogen receptor-positive breast cancer
    Article Snippet: The MCF-12A cells were cultured in 50% Ham’s F12 medium and 50% DMEM medium containing 5% horse serum, 1% antibiotic-antimycotic and 20 ng/mL epidermal growth factor (EGF), 10 μg/mL insulin, 500 ng/mL hydrocortisone and 100 ng/mL of cholera toxin.The MCF-12A cells were cultured in 50% Ham’s F12 medium and 50% DMEM medium containing 5% horse serum, 1% antibiotic-antimycotic and 20 ng/mL epidermal growth factor (EGF), 10 μg/mL insulin, 500 ng/mL hydrocortisone and 100 ng/mL of cholera toxin.. For experiments involving non-genetically manipulated human mammary epithelial cells (HuMECs) (Fig. ), human primary mammary epithelial cells were purchased from ATCC (HMEC, PCS-600-010).. These cells were grown in Mammary Epithelial Cell Basal Medium (ATCC) supplemented with the components of the Mammary Epithelial Cell Growth Kit (ATCC).These cells were grown in Mammary Epithelial Cell Basal Medium (ATCC) supplemented with the components of the Mammary Epithelial Cell Growth Kit (ATCC).

    Comparison:

    Article Title: The oncogene AAMDC links PI3K-AKT-mTOR signaling with metabolic reprograming in estrogen receptor-positive breast cancer
    Article Snippet: The MCF-12A cells were cultured in 50% Ham’s F12 medium and 50% DMEM medium containing 5% horse serum, 1% antibiotic-antimycotic and 20 ng/mL epidermal growth factor (EGF), 10 μg/mL insulin, 500 ng/mL hydrocortisone and 100 ng/mL of cholera toxin.The MCF-12A cells were cultured in 50% Ham’s F12 medium and 50% DMEM medium containing 5% horse serum, 1% antibiotic-antimycotic and 20 ng/mL epidermal growth factor (EGF), 10 μg/mL insulin, 500 ng/mL hydrocortisone and 100 ng/mL of cholera toxin.. For experiments involving non-genetically manipulated human mammary epithelial cells (HuMECs) (Fig. ), human primary mammary epithelial cells were purchased from ATCC (HMEC, PCS-600-010).. These cells were grown in Mammary Epithelial Cell Basal Medium (ATCC) supplemented with the components of the Mammary Epithelial Cell Growth Kit (ATCC).These cells were grown in Mammary Epithelial Cell Basal Medium (ATCC) supplemented with the components of the Mammary Epithelial Cell Growth Kit (ATCC).

    Immunocytochemistry:

    Article Title: The oncogene AAMDC links PI3K-AKT-mTOR signaling with metabolic reprograming in estrogen receptor-positive breast cancer
    Article Snippet: The MCF-12A cells were cultured in 50% Ham’s F12 medium and 50% DMEM medium containing 5% horse serum, 1% antibiotic-antimycotic and 20 ng/mL epidermal growth factor (EGF), 10 μg/mL insulin, 500 ng/mL hydrocortisone and 100 ng/mL of cholera toxin.The MCF-12A cells were cultured in 50% Ham’s F12 medium and 50% DMEM medium containing 5% horse serum, 1% antibiotic-antimycotic and 20 ng/mL epidermal growth factor (EGF), 10 μg/mL insulin, 500 ng/mL hydrocortisone and 100 ng/mL of cholera toxin.. For experiments involving non-genetically manipulated human mammary epithelial cells (HuMECs) (Fig. ), human primary mammary epithelial cells were purchased from ATCC (HMEC, PCS-600-010).. These cells were grown in Mammary Epithelial Cell Basal Medium (ATCC) supplemented with the components of the Mammary Epithelial Cell Growth Kit (ATCC).These cells were grown in Mammary Epithelial Cell Basal Medium (ATCC) supplemented with the components of the Mammary Epithelial Cell Growth Kit (ATCC).

    Transformation Assay:

    Article Title: The oncogene AAMDC links PI3K-AKT-mTOR signaling with metabolic reprograming in estrogen receptor-positive breast cancer
    Article Snippet: The MCF-12A cells were cultured in 50% Ham’s F12 medium and 50% DMEM medium containing 5% horse serum, 1% antibiotic-antimycotic and 20 ng/mL epidermal growth factor (EGF), 10 μg/mL insulin, 500 ng/mL hydrocortisone and 100 ng/mL of cholera toxin.The MCF-12A cells were cultured in 50% Ham’s F12 medium and 50% DMEM medium containing 5% horse serum, 1% antibiotic-antimycotic and 20 ng/mL epidermal growth factor (EGF), 10 μg/mL insulin, 500 ng/mL hydrocortisone and 100 ng/mL of cholera toxin.. For experiments involving non-genetically manipulated human mammary epithelial cells (HuMECs) (Fig. ), human primary mammary epithelial cells were purchased from ATCC (HMEC, PCS-600-010).. These cells were grown in Mammary Epithelial Cell Basal Medium (ATCC) supplemented with the components of the Mammary Epithelial Cell Growth Kit (ATCC).These cells were grown in Mammary Epithelial Cell Basal Medium (ATCC) supplemented with the components of the Mammary Epithelial Cell Growth Kit (ATCC).



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    a Analysis of somatic alterations of AAMDC using cancer genomic data sets and tools available from cBioPortal (see “Methods”). The frequency of amplification is shown as a percentage and the sample numbers are shown in brackets. METABRIC Molecular Taxonomy of Breast Cancer International Consortium, TCGA The Cancer Genome Atlas, BRCA Breast Cancer, INSERM Institut national de la santé et de la recherche médicale, MBC Metastatic Breast Cancer, NSCLC non-small-cell lung carcinoma, FHCRC Fred Hutchinson Cancer Research Center, NEPC National Environment Protection Council, PanCan Pan-Cancer. b Kaplan–Meier survival plots for patients with tumors expressing high (red) or low (green) levels of AAMDC mRNA. The lower left plots correspond to luminal B tumors treated with tamoxifen (see “Methods”). The p value shown for each plot is determined by the log-rank test. GEO Gene Expression Omnibus, GSE genomic spatial event, NSCLC non-small-cell lung carcinoma. c Localization of the AAMDC protein in tumors from a breast tissue microarray (TMA) assessed by immunohistochemistry (IHC). Representative IHC sections of normal breast tissue, estrogen receptor-negative (ER − ) tumor tissue, ductal carcinoma in situ (DCIS), and invasive ductal carcinoma (IDC) are shown. 0, 1+, 2+, 3+ indicate the staining intensity score. d Associations between AAMDC expression (IHC) and lymph node metastasis (LN + ) as well as tumor grade, tumor size (T3-4), and ER positivity (ER + ) by AAMDC localization from the same TMA. Statistical significance is indicated by Chi-square analysis with a one-tailed p -value relative to ER − tissue. For T3-4: * p = 0.03; for LN + : * p = 0.03; for ER + , from left to right: * p = 0.003, * p = 0.005, * p = 0.005. n = 60 biologically independent samples. Full details of the TMA are provided in Supplementary Table . e Frequency of AAMDC amplification/polysomy in a cohort of 119 luminal B breast cancer specimens. Representative fluorescence in situ hybridization (FISH) images are indicated, with specific probes for AAMDC (red) and Centromere enumeration 11 probe for chromosome 11 ( C11 , green). The full clinical and pathological features of these tumors are shown in Supplementary Data . f Real-time expression analyses (qRT-PCR) of AAMDC in luminal, non-luminal, and normal-like breast cells. Significance levels are determined relative to MCF-12A by Ordinary one-way ANOVA with Dunnett multiple comparison test. Data are presented as mean values ± SEM (* p = 0.0217, ** p = 0.0018, **** p < 0.0001). n = 3 biologically independent RNA extractions. Representative images of immunocytochemistry (ICC) and FISH of selected luminal cell lines are presented. <t>HuMECs</t> non-transformed human mammary <t>epithelial</t> cells.
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    Image Search Results


    a Analysis of somatic alterations of AAMDC using cancer genomic data sets and tools available from cBioPortal (see “Methods”). The frequency of amplification is shown as a percentage and the sample numbers are shown in brackets. METABRIC Molecular Taxonomy of Breast Cancer International Consortium, TCGA The Cancer Genome Atlas, BRCA Breast Cancer, INSERM Institut national de la santé et de la recherche médicale, MBC Metastatic Breast Cancer, NSCLC non-small-cell lung carcinoma, FHCRC Fred Hutchinson Cancer Research Center, NEPC National Environment Protection Council, PanCan Pan-Cancer. b Kaplan–Meier survival plots for patients with tumors expressing high (red) or low (green) levels of AAMDC mRNA. The lower left plots correspond to luminal B tumors treated with tamoxifen (see “Methods”). The p value shown for each plot is determined by the log-rank test. GEO Gene Expression Omnibus, GSE genomic spatial event, NSCLC non-small-cell lung carcinoma. c Localization of the AAMDC protein in tumors from a breast tissue microarray (TMA) assessed by immunohistochemistry (IHC). Representative IHC sections of normal breast tissue, estrogen receptor-negative (ER − ) tumor tissue, ductal carcinoma in situ (DCIS), and invasive ductal carcinoma (IDC) are shown. 0, 1+, 2+, 3+ indicate the staining intensity score. d Associations between AAMDC expression (IHC) and lymph node metastasis (LN + ) as well as tumor grade, tumor size (T3-4), and ER positivity (ER + ) by AAMDC localization from the same TMA. Statistical significance is indicated by Chi-square analysis with a one-tailed p -value relative to ER − tissue. For T3-4: * p = 0.03; for LN + : * p = 0.03; for ER + , from left to right: * p = 0.003, * p = 0.005, * p = 0.005. n = 60 biologically independent samples. Full details of the TMA are provided in Supplementary Table . e Frequency of AAMDC amplification/polysomy in a cohort of 119 luminal B breast cancer specimens. Representative fluorescence in situ hybridization (FISH) images are indicated, with specific probes for AAMDC (red) and Centromere enumeration 11 probe for chromosome 11 ( C11 , green). The full clinical and pathological features of these tumors are shown in Supplementary Data . f Real-time expression analyses (qRT-PCR) of AAMDC in luminal, non-luminal, and normal-like breast cells. Significance levels are determined relative to MCF-12A by Ordinary one-way ANOVA with Dunnett multiple comparison test. Data are presented as mean values ± SEM (* p = 0.0217, ** p = 0.0018, **** p < 0.0001). n = 3 biologically independent RNA extractions. Representative images of immunocytochemistry (ICC) and FISH of selected luminal cell lines are presented. HuMECs non-transformed human mammary epithelial cells.

    Journal: Nature Communications

    Article Title: The oncogene AAMDC links PI3K-AKT-mTOR signaling with metabolic reprograming in estrogen receptor-positive breast cancer

    doi: 10.1038/s41467-021-22101-7

    Figure Lengend Snippet: a Analysis of somatic alterations of AAMDC using cancer genomic data sets and tools available from cBioPortal (see “Methods”). The frequency of amplification is shown as a percentage and the sample numbers are shown in brackets. METABRIC Molecular Taxonomy of Breast Cancer International Consortium, TCGA The Cancer Genome Atlas, BRCA Breast Cancer, INSERM Institut national de la santé et de la recherche médicale, MBC Metastatic Breast Cancer, NSCLC non-small-cell lung carcinoma, FHCRC Fred Hutchinson Cancer Research Center, NEPC National Environment Protection Council, PanCan Pan-Cancer. b Kaplan–Meier survival plots for patients with tumors expressing high (red) or low (green) levels of AAMDC mRNA. The lower left plots correspond to luminal B tumors treated with tamoxifen (see “Methods”). The p value shown for each plot is determined by the log-rank test. GEO Gene Expression Omnibus, GSE genomic spatial event, NSCLC non-small-cell lung carcinoma. c Localization of the AAMDC protein in tumors from a breast tissue microarray (TMA) assessed by immunohistochemistry (IHC). Representative IHC sections of normal breast tissue, estrogen receptor-negative (ER − ) tumor tissue, ductal carcinoma in situ (DCIS), and invasive ductal carcinoma (IDC) are shown. 0, 1+, 2+, 3+ indicate the staining intensity score. d Associations between AAMDC expression (IHC) and lymph node metastasis (LN + ) as well as tumor grade, tumor size (T3-4), and ER positivity (ER + ) by AAMDC localization from the same TMA. Statistical significance is indicated by Chi-square analysis with a one-tailed p -value relative to ER − tissue. For T3-4: * p = 0.03; for LN + : * p = 0.03; for ER + , from left to right: * p = 0.003, * p = 0.005, * p = 0.005. n = 60 biologically independent samples. Full details of the TMA are provided in Supplementary Table . e Frequency of AAMDC amplification/polysomy in a cohort of 119 luminal B breast cancer specimens. Representative fluorescence in situ hybridization (FISH) images are indicated, with specific probes for AAMDC (red) and Centromere enumeration 11 probe for chromosome 11 ( C11 , green). The full clinical and pathological features of these tumors are shown in Supplementary Data . f Real-time expression analyses (qRT-PCR) of AAMDC in luminal, non-luminal, and normal-like breast cells. Significance levels are determined relative to MCF-12A by Ordinary one-way ANOVA with Dunnett multiple comparison test. Data are presented as mean values ± SEM (* p = 0.0217, ** p = 0.0018, **** p < 0.0001). n = 3 biologically independent RNA extractions. Representative images of immunocytochemistry (ICC) and FISH of selected luminal cell lines are presented. HuMECs non-transformed human mammary epithelial cells.

    Article Snippet: For experiments involving non-genetically manipulated human mammary epithelial cells (HuMECs) (Fig. ), human primary mammary epithelial cells were purchased from ATCC (HMEC, PCS-600-010).

    Techniques: Amplification, Expressing, Gene Expression, Microarray, Immunohistochemistry, In Situ, Staining, One-tailed Test, Fluorescence, In Situ Hybridization, Quantitative RT-PCR, Comparison, Immunocytochemistry, Transformation Assay